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Modified Listeria bacteriophage lysin genes (ply) allow efficient overexpression and one-step purification of biochemically active fusion proteins.

Listeria bacteriophage lytic enzymes are useful for in vitro applications such as rapid, gentle cell disruption, and they provide new approaches as selective antimicrobial agents for destruction of Listeria monocytogenes in contaminated foods. We describe here the amino-terminal modification of thre...

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Détails bibliographiques
Publié dans:Appl Environ Microbiol
Auteurs principaux: Loessner, M J, Schneider, A, Scherer, S
Format: Artigo
Langue:Inglês
Publié: American Society for Microbiology (ASM) 1996
Sujets:
Accès en ligne:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC168095/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8702301/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.62.8.3057-3060.1996
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