Rapid purification of the oxygenase component of toluene dioxygenase from a polyol-responsive monoclonal antibody.

A monoclonal antibody designated 302 beta that is specific for the beta subunit of the oxygenase component (ISPTOL) of toluene dioxygenase from Pseudomonas putida F1 was used to prepare an immunoaffinity column. ISPTOL in cell extracts of Escherichia coli JM109(pDTG611) bound to the column, and an e...

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Detalhes bibliográficos

Publicado no:	Appl Environ Microbiol
Principais autores:	Lynch, N A, Jiang, H, Gibson, D T
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1996
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC167991/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8787410/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.62.6.2133-2137.1996
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