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Characterization of a restriction-modification system of the thermotolerant methylotroph Bacillus methanolicus.

We report the isolation of a restriction endonuclease, BmeTI, an isoschizomer of BclI, that recognizes the DNA sequence 5' TGATCA 3'. We also report that BmeTI sites are modified to TGm6ATCA. These findings provide the basis for devising strategies to prevent BmeTI restriction of any DNA introduced...

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Bibliografiske detaljer
Udgivet i:Appl Environ Microbiol
Principais autores: Cue, D, Lam, H, Hanson, R S, Flickinger, M C
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 1996
Fag:
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC167875/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8975604/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.62.3.1107-1111.1996
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