Bias caused by template annealing in the amplification of mixtures of 16S rRNA genes by PCR.
The PCR is used widely for the study of rRNA genes amplified from mixed microbial populations. These studies resemble quantitative applications of PCR in that the templates are mixtures of homologs and the relative abundance of amplicons is thought to provide some measure of the gene ratios in the s...
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| Udgivet i: | Appl Environ Microbiol |
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| Principais autores: | , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
American Society for Microbiology (ASM)
1996
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC167828/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8593063/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.62.2.625-630.1996 |
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