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DNA recovery from soils of diverse composition.

A simple, rapid method for bacterial lysis and direct extraction of DNA from soils with minimal shearing was developed to address the risk of chimera formation from small template DNA during subsequent PCR. The method was based on lysis with a high-salt extraction buffer (1.5 M NaCl) and extended he...

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Podrobná bibliografie
Hlavní autoři: Zhou, J, Bruns, M A, Tiedje, J M
Médium: Artigo
Jazyk:Inglês
Vydáno: 1996
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC167800/
https://ncbi.nlm.nih.gov/pubmed/8593035
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