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DNA recovery from soils of diverse composition.

A simple, rapid method for bacterial lysis and direct extraction of DNA from soils with minimal shearing was developed to address the risk of chimera formation from small template DNA during subsequent PCR. The method was based on lysis with a high-salt extraction buffer (1.5 M NaCl) and extended he...

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Bibliografische gegevens
Hoofdauteurs: Zhou, J, Bruns, M A, Tiedje, J M
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: 1996
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC167800/
https://ncbi.nlm.nih.gov/pubmed/8593035
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