A new method for direct detection of Listeria monocytogenes from foods by PCR.
The preparation of DNA by alcohol precipitation in the presence of Na1 was used to enable the direct detection of Listeria monocytogenes in the amount of 10(3) CFU/0.5 g of sample. This procedure produces PCR-quality DNA directly from foods, such as soft cheese and minced meat.
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| Publicado no: | Appl Environ Microbiol |
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| Principais autores: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Microbiology (ASM)
1995
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC167673/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7487010/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.61.10.3745-3747.1995 |
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