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Microscale detection of specific bacterial DNA in soil with a magnetic capture-hybridization and PCR amplification assay.

A magnetic capture-hybridization PCR technique (MCH-PCR) was developed to eliminate the inhibitory effect of humic acids and other contaminants in PCRs targeting specific soil DNA. A single-stranded DNA probe, which was complementary to an internal part of the target gene, was used to coat magnetic...

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Bibliografske podrobnosti
Glavni avtor: Jacobsen, C S
Format: Artigo
Jezik:Inglês
Izdano: 1995
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC167615/
https://ncbi.nlm.nih.gov/pubmed/7574645
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