Effect of genome size and rrn gene copy number on PCR amplification of 16S rRNA genes from a mixture of bacterial species.
In order to assess the effect of genome size and number of 16S rRNA genes (rDNAs) on the quantities of PCR-generated partial 16S rDNA fragments, equimolar amounts of DNA from pairs of different species for which these parameters are known were subjected to gene amplification. The experimentally dete...
Enregistré dans:
| Publié dans: | Appl Environ Microbiol |
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| Auteurs principaux: | , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
American Society for Microbiology (ASM)
1995
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC167554/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7618894/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.61.7.2798-2801.1995 |
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