Gene cloning, sequence analysis, purification, and secretion by Escherichia coli of an extracellular lipase from Serratia marcescens.

The gene encoding extracellular lipase of Serratia marcescens has been identified from a phage lambda genomic library. Formation of orange-red fluorescent plaques on rhodamine B-triolein plates was used to identify phages carrying the lipase gene. A 2.8-kb SalI fragment was subcloned into a plasmid,...

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Detalhes bibliográficos

Publicado no:	Appl Environ Microbiol
Principais autores:	Li, X, Tetling, S, Winkler, U K, Jaeger, K E, Benedik, M J
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1995
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC167541/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7618881/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.61.7.2674-2680.1995
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