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Construction and use of a nontoxigenic strain of Pseudomonas aeruginosa for the production of recombinant exotoxin A.

To express recombinant forms of Pseudomonas aeruginosa exotoxin A in high yield, we have developed a nontoxigenic strain of P. aeruginosa derived from the hypertoxigenic strain PA103. The nontoxigenic strain, designated PA103A, was produced by the excision marker rescue technique to replace the toxA...

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Udgivet i:Appl Environ Microbiol
Principais autores: Wozniak, D J, Han, X Y, Galloway, D R
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 1995
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC167436/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7646011/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.61.5.1739-1744.1995
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