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Analysis of viral RNA persistence in seawater by reverse transcriptase-PCR.

It is important to determine the stability of naked viral RNA in seawater, since false-positive results can occur when reverse transcriptase-PCR (RT-PCR) is used to detect viruses if the RT-PCR amplifies free RNA instead of RNA from intact viruses. An acid guanidinium thiocyanate-phenol-chloroform m...

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Bibliografische gegevens
Hoofdauteurs: Tsai, Y L, Tran, B, Palmer, C J
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: 1995
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC167290/
https://ncbi.nlm.nih.gov/pubmed/7887616
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