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De Novo Synthesis of Negative-Strand RNA by Dengue Virus RNA-Dependent RNA Polymerase In Vitro: Nucleotide, Primer, and Template Parameters

By using a purified dengue virus RNA-dependent RNA polymerase and a subgenomic 770-nucleotide RNA template, it was shown previously that the ratio of the de novo synthesis product to hairpin product formed was inversely proportional to increments of assay temperatures (20 to 40°C). In this study, th...

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Autors principals: Nomaguchi, Masako, Ackermann, Matt, Yon, Changsuek, You, Shihyun, Padmanbhan, R.
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology 2003
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC167251/
https://ncbi.nlm.nih.gov/pubmed/12885902
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JVI.77.16.8831-8842.2003
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