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De Novo Synthesis of Negative-Strand RNA by Dengue Virus RNA-Dependent RNA Polymerase In Vitro: Nucleotide, Primer, and Template Parameters

By using a purified dengue virus RNA-dependent RNA polymerase and a subgenomic 770-nucleotide RNA template, it was shown previously that the ratio of the de novo synthesis product to hairpin product formed was inversely proportional to increments of assay temperatures (20 to 40°C). In this study, th...

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Detalhes bibliográficos
Publicado no:J Virol
Principais autores: Nomaguchi, Masako, Ackermann, Matt, Yon, Changsuek, You, Shihyun, Padmanbhan, R.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 2003
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC167251/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12885902/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JVI.77.16.8831-8842.2003
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