hpRNA-Mediated Targeting of the Arabidopsis FAD2 Gene Gives Highly Efficient and Stable Silencing

The endogenous Δ12-desaturase gene (FAD2) in Arabidopsis was targeted for silencing using seed-specific cosuppression (CS), hairpin (HP) RNA (hpRNA), and intron-spliced HP (iHP) constructs. The iHP construct, incorporating the 120-bp 3′-untranslated region of the FAD2 gene, gave the highest degree o...

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Main Authors: Stoutjesdijk, Peter A., Singh, Surinder P., Liu, Qing, Hurlstone, Clive J., Waterhouse, Peter A., Green, Allan G.
Formato: Artigo
Idioma:Inglês
Publicado: American Society of Plant Physiologists 2002
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Research Article
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC166760/
https://ncbi.nlm.nih.gov/pubmed/12177485
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1104/pp.006353
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