Conversion of Lactobacillus pentosus d-Lactate Dehydrogenase to a d-Hydroxyisocaproate Dehydrogenase through a Single Amino Acid Replacement

The single amino acid replacement of Tyr52 with Leu drastically increased the activity of Lactobacillus pentosus NAD-dependent d-lactate dehydrogenase toward larger aliphatic or aromatic 2-ketoacid substrates by 3 or 4 orders of magnitude and decreased the activity toward pyruvate by about 30-fold,...

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Autors principals: Tokuda, Chizuka, Ishikura, Yoshiro, Shigematsu, Mayu, Mutoh, Hiroyuki, Tsuzuki, Shino, Nakahira, Yusaku, Tamura, Yusuke, Shinoda, Takeshi, Arai, Kazuhito, Takahashi, O, Taguchi, Hayao
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology 2003
Matèries:
Enzymes and Proteins
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC166478/
https://ncbi.nlm.nih.gov/pubmed/12897026
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.185.16.5023-5026.2003
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