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The N-terminal domains of histones H3 and H4 are not necessary for chromatin assembly factor-1- mediated nucleosome assembly onto replicated DNA in vitro

An in vitro reconstitution system for the analysis of replication-coupled nucleosome assembly is described. In this “two-step system,” nucleosome assembly is performed in a separate reaction from DNA replication, wherein purified newly replicated DNA remains noncovalently marked for subsequent chrom...

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Bibliografiske detaljer
Main Authors: Shibahara, Kei-ichi, Verreault, Alain, Stillman, Bruce
Format: Artigo
Sprog:Inglês
Udgivet: The National Academy of Sciences 2000
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC16619/
https://ncbi.nlm.nih.gov/pubmed/10884407
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