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The frequency of gene targeting in Trypanosoma brucei is independent of target site copy number

We have investigated the effect of target copy number on the efficiency of stable transformation of the protozoan parasite Trypanosoma brucei. Using a single strain of the organism, we targeted integrative vectors to several different genomic sequences, occurring at copy numbers ranging from 1 to ∼3...

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Detalhes bibliográficos
Main Authors: Wickstead, Bill, Ersfeld, Klaus, Gull, Keith
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2003
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC165960/
https://ncbi.nlm.nih.gov/pubmed/12853615
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