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An optimized grapevine RNA isolation procedure and statistical determination of reference genes for real-time RT-PCR during berry development
BACKGROUND: Accuracy in quantitative real-time RT-PCR is dependent on high quality RNA, consistent cDNA synthesis, and validated stable reference genes for data normalization. Reference genes used for normalization impact the results generated from expression studies and, hence, should be evaluated...
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| Auteurs principaux: | , , , , |
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| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
BioMed Central
2006
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1654153/ https://ncbi.nlm.nih.gov/pubmed/17105665 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2229-6-27 |
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