Real-time light-driven dynamics of the fluorescence emission in single green fluorescent protein molecules

Real-time single-molecule fluorescence detection using confocal and near-field scanning optical microscopy has been applied to elucidate the nature of the “on–off” blinking observed in the Ser-65 → Thr (S65T) mutant of the green fluorescent protein (GFP). Fluorescence time traces as a function of th...

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Detalhes bibliográficos

Publicado no:	Proc Natl Acad Sci U S A
Principais autores:	Garcia-Parajo, M. F., Segers-Nolten, G. M. J., Veerman, J.-A., Greve, J., van Hulst, N. F.
Formato:	Artigo
Idioma:	Inglês
Publicado em:	National Academy of Sciences 2000
Assuntos:	Biological Sciences
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC16529/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10860989/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.97.13.7237
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