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piggyBac-based insertional mutagenesis in the presence of stably integrated P elements in Drosophila
P element-mediated mutagenesis has been used to disrupt an estimated 25% of genes essential for Drosophila adult viability. Mutation of all genes in the fly genome, however, poses a problem, because P elements show significant hotspots of integration. In addition, advanced screening scenarios often...
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| Main Authors: | , , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
National Academy of Sciences
2003
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC164654/ https://ncbi.nlm.nih.gov/pubmed/12802016 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1230526100 |
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