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In Vitro Synthesis of Minus-Strand RNA by an Isolated Cereal Yellow Dwarf Virus RNA-Dependent RNA Polymerase Requires VPg and a Stem-Loop Structure at the 3′ End of the Virus RNA

Cereal yellow dwarf virus (CYDV) RNA has a 5′-terminal genome-linked protein (VPg). We have expressed the VPg region of the CYDV genome in bacteria and used the purified protein (bVPg) to raise an antiserum which was able to detect free VPg in extracts of CYDV-infected oat plants. A template-depende...

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Bibliografske podrobnosti
Main Authors: Osman, Toba A. M., Coutts, Robert H. A., Buck, Kenneth W.
Format: Artigo
Jezik:Inglês
Izdano: American Society for Microbiology 2006
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC1641740/
https://ncbi.nlm.nih.gov/pubmed/16928757
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JVI.01050-06
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