Microarray scanner calibration curves: characteristics and implications

BACKGROUND: Microarray-based measurement of mRNA abundance assumes a linear relationship between the fluorescence intensity and the dye concentration. In reality, however, the calibration curve can be nonlinear. RESULTS: By scanning a microarray scanner calibration slide containing known concentrati...

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Bibliographic Details
Main Authors: Shi, Leming, Tong, Weida, Su, Zhenqiang, Han, Tao, Han, Jing, Puri, Raj K, Fang, Hong, Frueh, Felix W, Goodsaid, Federico M, Guo, Lei, Branham, William S, Chen, James J, Xu, Z Alex, Harris, Stephen C, Hong, Huixiao, Xie, Qian, Perkins, Roger G, Fuscoe, James C
Format: Artigo
Language:Inglês
Published: BioMed Central 2005
Subjects:
Proceedings
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC1637029/
https://ncbi.nlm.nih.gov/pubmed/16026596
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2105-6-S2-S11
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