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The Relaxase of the Rhizobium etli Symbiotic Plasmid Shows nic Site cis-Acting Preference
Genetic and biochemical characterization of TraA, the relaxase of symbiotic plasmid pRetCFN42d from Rhizobium etli, is described. After purifying the relaxase domain (N265TraA), we demonstrated nic binding and cleavage activity in vitro and thus characterized for the first time the nick site (nic) o...
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| Главные авторы: | , , , , , , |
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| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
American Society for Microbiology
2006
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1636270/ https://ncbi.nlm.nih.gov/pubmed/16916896 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.00701-06 |
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