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Construction of a Library of Human Glycosyltransferases Immobilized in the Cell Wall of Saccharomyces cerevisiae

Fifty-one human glycosyltransferases were expressed in Saccharomyces cerevisiae as immobilized enzymes and were assayed for enzymatic activities. The stem and catalytic regions of sialyl-, fucosyl-, galactosyl-, N-acetylgalactosaminyl-, and N-acetylglucosaminyltransferases were fused with yeast cell...

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Detalhes bibliográficos
Main Authors: Shimma, Yoh-ichi, Saito, Fumie, Oosawa, Fumi, Jigami, Yoshifumi
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2006
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1636194/
https://ncbi.nlm.nih.gov/pubmed/16936046
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.01378-06
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