Effects of Mutations in the Substrate-Binding Domain of Poly[(R)-3-Hydroxybutyrate] (PHB) Depolymerase from Ralstonia pickettii T1 on PHB Degradation

Poly[(R)-3-hydroxybutyrate] (PHB) depolymerase from Ralstonia pickettii T1 (PhaZ(RpiT1)) adsorbs to denatured PHB (dPHB) via its substrate-binding domain (SBD) to enhance dPHB degradation. To evaluate the amino acid residues participating in dPHB adsorption, PhaZ(RpiT1) was subjected to a high-throu...

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Detalhes bibliográficos
Main Authors: Hiraishi, Tomohiro, Hirahara, Yoko, Doi, Yoshiharu, Maeda, Mizuo, Taguchi, Seiichi
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2006
Assuntos:
Enzymology and Protein Engineering
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1636158/
https://ncbi.nlm.nih.gov/pubmed/16963553
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.01187-06
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