Application of Pseudomurein Endoisopeptidase to Fluorescence In Situ Hybridization of Methanogens within the Family Methanobacteriaceae

In situ detection of methanogens within the family Methanobacteriaceae is sometimes known to be unsuccessful due to the difficulty in permeability of oligonucleotide probes. Pseudomurein endoisopeptidase (Pei), a lytic enzyme that specifically acts on their cell walls, was applied prior to 16S rRNA-...

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Main Authors: Nakamura, Kohei , Terada, Takeshi, Sekiguchi, Yuji, Shinzato, Naoya, Meng, Xian-Ying, Enoki, Miho, Kamagata, Yoichi
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2006
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Methods
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1636154/
https://ncbi.nlm.nih.gov/pubmed/16950902
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.01499-06
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