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Identification of pathogenic Leptospira species by conventional or real-time PCR and sequencing of the DNA gyrase subunit B encoding gene

BACKGROUND: Leptospira is the causative genus of the disease, leptospirosis. Species identification of pathogenic Leptospira in the past was generally performed by either DNA-DNA hybridisation or 16s rRNA gene sequencing. Both methods have inherent disadvantages such as the need for radio-labelled i...

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Autores principales: Slack, Andrew T, Symonds, Meegan L, Dohnt, Michael F, Smythe, Lee D
Formato: Artigo
Lenguaje:Inglês
Publicado: BioMed Central 2006
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC1630700/
https://ncbi.nlm.nih.gov/pubmed/17067399
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2180-6-95
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