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Bioluminescence method to evaluate antimicrobial agents against Mycobacterium avium.

Plasmid pLUC10, carrying the firefly luciferase gene, was transformed by electroporation into Mycobacterium avium A5. Bioluminescence production by strain A5(pLUC10), as measured in a microdilution plate luminometer, was approximately 1 relative light unit per 2 x 10(6) viable bacilli, whereas it wa...

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Bibliographic Details
Main Authors: Cooksey, R C, Morlock, G P, Beggs, M, Crawford, J T
Format: Artigo
Language:Inglês
Published: 1995
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC162618/
https://ncbi.nlm.nih.gov/pubmed/7793886
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