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Application of 4,5-diaminofluorescein to reliably measure nitric oxide released from endothelial cells in vitro

Here we describe in more depth the previously published application of the fluorescent probe 4,5-diaminofluorescein (DAF-2) in order to reliably measure low levels of nitric oxide (NO) as released from human endothelial cells in vitro. The used approach is based on the following considerations a) us...

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Detalhes bibliográficos
Main Authors: Räthel, Thomas R., Leikert, Jürgen F., Vollmar, Angelika M., Dirsch, Verena M.
Formato: Artigo
Idioma:Inglês
Publicado em: Biological Procedures Online 2003
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC162170/
https://ncbi.nlm.nih.gov/pubmed/14569611
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1251/bpo55
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