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Lateral Mobility of Membrane-Binding Proteins in Living Cells Measured by Total Internal Reflection Fluorescence Correlation Spectroscopy

Total internal reflection fluorescence correlation spectroscopy (TIR-FCS) allows us to measure diffusion constants and the number of fluorescent molecules in a small area of an evanescent field generated on the objective of a microscope. The application of TIR-FCS makes possible the characterization...

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Detalhes bibliográficos
Main Authors: Ohsugi, Yu, Saito, Kenta, Tamura, Mamoru, Kinjo, Masataka
Formato: Artigo
Idioma:Inglês
Publicado em: Biophysical Society 2006
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1614500/
https://ncbi.nlm.nih.gov/pubmed/16891361
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.105.074625
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