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High-Resolution AFM of Membrane Proteins Directly Incorporated at High Density in Planar Lipid Bilayer

The heterologous expression and purification of membrane proteins represent major limitations for their functional and structural analysis. Here we describe a new method of incorporation of transmembrane proteins in planar lipid bilayer starting from 1 pmol of solubilized proteins. The principle rel...

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Detalhes bibliográficos
Main Authors: Milhiet, Pierre-Emmanuel, Gubellini, Francesca, Berquand, Alexandre, Dosset, Patrice, Rigaud, Jean-Louis, Le Grimellec, Christian, Lévy, Daniel
Formato: Artigo
Idioma:Inglês
Publicado em: Biophysical Society 2006
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1614478/
https://ncbi.nlm.nih.gov/pubmed/16905620
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.106.087791
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