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High-Resolution AFM of Membrane Proteins Directly Incorporated at High Density in Planar Lipid Bilayer

The heterologous expression and purification of membrane proteins represent major limitations for their functional and structural analysis. Here we describe a new method of incorporation of transmembrane proteins in planar lipid bilayer starting from 1 pmol of solubilized proteins. The principle rel...

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Autores principales: Milhiet, Pierre-Emmanuel, Gubellini, Francesca, Berquand, Alexandre, Dosset, Patrice, Rigaud, Jean-Louis, Le Grimellec, Christian, Lévy, Daniel
Formato: Artigo
Lenguaje:Inglês
Publicado: Biophysical Society 2006
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC1614478/
https://ncbi.nlm.nih.gov/pubmed/16905620
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.106.087791
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