Regeneration of Fertile Barley Plants from Mechanically Isolated Protoplasts of the Fertilized Egg Cell.

A simple procedure is described for the mechanical isolation of protoplasts of unfertilized and fertilized barley egg cells from dissected ovules. Viable protoplasts were isolated from ~75% of the dissected ovules. Unfertilized protoplasts did not divide, whereas almost all fertilized protoplasts de...

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Detalhes bibliográficos

Publicado no:	Plant Cell
Principais autores:	Holm, P. B., Knudsen, S., Mouritzen, P., Negri, D., Olsen, F. L., Roue, C.
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Oxford University Press 1994
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC160456/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12244247/ https://ncbi.nlm.nih.govhttps://doi.org/10.1105/tpc.6.4.531
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