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Preparation of His-Tagged Armored RNA Phage Particles as a Control for Real-Time Reverse Transcription-PCR Detection of Severe Acute Respiratory Syndrome Coronavirus
Armored RNA has been increasingly used as both an external and internal positive control in nucleic acid-based assays for RNA virus. In order to facilitate armored RNA purification, a His(6) tag was introduced into the loop region of the MS2 coat protein, which allows the exposure of multiple His ta...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Microbiology
2006
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1594775/ https://ncbi.nlm.nih.gov/pubmed/17021082 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.00713-06 |
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