Optimization and Validation of Recombinant Serological Tests for African Swine Fever Diagnosis Based on Detection of the p30 Protein Produced in Trichoplusia ni Larvae

We describe the validation of an enzyme-linked immunosorbent assay (ELISA) and confirmatory immunoblotting assays based on a recombinant p30 protein (p30r) produced in insect larvae using a baculovirus vector. Such validation included the following: (i) the scaling up and standardization of p30r pro...

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Detalhes bibliográficos
Main Authors: Pérez-Filgueira, D. M., González-Camacho, F., Gallardo, C., Resino-Talaván, P., Blanco, E., Gómez-Casado, E., Alonso, C., Escribano, J. M.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2006
Assuntos:
Clinical Veterinary Microbiology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1594705/
https://ncbi.nlm.nih.gov/pubmed/16954235
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.00406-06
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