Real-Time Quantitative Broad-Range PCR Assay for Detection of the 16S rRNA Gene Followed by Sequencing for Species Identification

Here we determined the analytical sensitivities of broad-range real-time PCR-based assays employing one of three different genomic DNA extraction protocols in combination with one of three different primer pairs targeting the 16S rRNA gene to detect a panel of 22 bacterial species. DNA extraction pr...

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Detalhes bibliográficos
Main Authors: Zucol, Franziska, Ammann, Roland A., Berger, Christoph, Aebi, Christoph, Altwegg, Martin, Niggli, Felix K., Nadal, David
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2006
Assuntos:
Bacteriology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1594602/
https://ncbi.nlm.nih.gov/pubmed/16891488
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.00112-06
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