Purification and Kinetic Properties of Serine Acetyltransferase Free of O-Acetylserine(thiol)lyase from Spinach Chloroplasts.

Serine acetyltransferase, a key enzyme in the L-cysteine biosynthetic pathway, was purified over 300,000-fold from the stroma of spinach (Spinacia oleracea) leaf chloroplasts. The purification procedure consisted of ammonium sulfate precipitation, anion-exchange chromatography (Trisacryl M DEAE and...

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發表在:	Plant Physiol
Principais autores:	Ruffet, M. L., Droux, M., Douce, R.
格式:	Artigo
語言:	Inglês
出版:	Oxford University Press 1994
主題:	Research Article
在線閱讀:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC159236/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12232109/ https://ncbi.nlm.nih.govhttps://doi.org/10.1104/pp.104.2.597
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