Identification of the uridine-binding domain of sucrose-phosphate synthase. Expression of a region of the protein that photoaffinity labels with 5-azidouridine diphosphate-glucose.

The uridine diphosphate-glucose (UDP-Glc) binding domain of sucrose-phosphate synthase (SPS) was identified by overexpressing part of the gene from spinach (Spinacia oleracea). Degenerate oligonucleotide primers corresponding to two tryptic peptides common to both the full-length 120-kD SPS subunit...

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Veröffentlicht in:	Plant Physiol
Hauptverfasser:	Salvucci, M E, Klein, R R
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	Oxford University Press 1993
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC158808/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8108511/ https://ncbi.nlm.nih.govhttps://doi.org/10.1104/pp.102.2.529
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