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Visualizing Enzyme Secretion from Individual Barley (Hordeum vulgare) Aleurone Protoplasts.

A method was developed to detect [alpha]-amylase gene expression and [alpha]-amylase secretion from individual barley (Hordeum vulgare L. cv Himalaya) aleurone protoplasts. Protoplasts are incubated in liquid media with or without hormones and embedded in a thin film of agarose and starch, where the...

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Bibliographic Details
Published in:Plant Physiol
Main Authors: Hillmer, S., Gilroy, S., Jones, R. L.
Format: Artigo
Language:Inglês
Published: Oxford University Press 1993
Subjects:
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC158773/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12231819/
https://ncbi.nlm.nih.govhttps://doi.org/10.1104/pp.102.1.279
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