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Analysis of wild-type and mutant plant nitrate reductase expressed in the methylotrophic yeast Pichia pastoris.

Recombinant Arabidopsis thaliana NADH:nitrate reductase (NR; EC 1.6.6.1) was produced in the methylotrophic yeast Pichia pastoris and purified to near-electrophoretic homogeneity. Purified enzyme had the spectral and kinetic properties typical of highly purified NR from natural plant sources. Site-d...

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Udgivet i:Plant Physiol
Principais autores: Su, W, Mertens, J A, Kanamaru, K, Campbell, W H, Crawford, N M
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 1997
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC158578/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9390442/
https://ncbi.nlm.nih.govhttps://doi.org/10.1104/pp.115.3.1135
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