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The Kinetics of N-Ethylmaleimide Inhibition of a Vacuolar H+-ATPase and Determination of Nucleotide Dissociation Constants.

All eukaryotic vacuolar (V-type) ATPases share the property of being inhibited by low concentrations (1-2 [mu]M) if N-ethylmaleimide (NEM). This distinguishes them from P-type ATPases, which are inhibited by higher concentrations of NEM (0.1-1 mM), and F-type ATPases, which are virtually resistant t...

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Publicado en:Plant Physiol
Autores principales: Hunt, I. E., Sanders, D.
Formato: Artigo
Lenguaje:Inglês
Publicado: Oxford University Press 1996
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Acceso en línea:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC157698/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12226173/
https://ncbi.nlm.nih.govhttps://doi.org/10.1104/pp.110.1.97
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