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Direct visualization of ligand-protein interactions using atomic force microscopy
1. Streptavidin is a 60-kDa tetramer which binds four molecules of biotin with extremely high affinity (K(A)∼10(14) M(−1)). We have used atomic force microscopy (AFM) to visualize this ligand-protein interaction directly. 2. Biotin was tagged with a short (152-basepair; 50-nm) DNA rod and incubated...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2002
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1573323/ https://ncbi.nlm.nih.gov/pubmed/11959797 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/sj.bjp.0704660 |
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