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Complementarity of ensemble and single-molecule measures of protein motion: A relaxation dispersion NMR study of an enzyme complex

Single-molecule fluorescence experiments have shown that the conformation of the complex between Escherichia coli general NAD(P)H:flavin oxidoreductase (FRE) and flavin adenine dinucleotide (FAD) fluctuates over a range of timescales between 10(−4) and 1 s. Here we use (15)N and (13)C relaxation dis...

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Detalhes bibliográficos
Main Authors: Vallurupalli, Pramodh, Kay, Lewis E.
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2006
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1567672/
https://ncbi.nlm.nih.gov/pubmed/16880391
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0602310103
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