Rapid Diagnosis of Extrapulmonary Tuberculosis by PCR: Impact of Sample Preparation and DNA Extraction

In cases of suspected extrapulmonary tuberculosis, rapid and accurate laboratory diagnosis is of prime importance, since traditional techniques of detecting acid-fast bacilli have limitations. The major difficulty with mycobacteria is achieving optimal cell lysis. Buffers used in commercial kits do...

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Bibliografiske detaljer
Main Authors: Honoré-Bouakline, S., Vincensini, J. P., Giacuzzo, V., Lagrange, P. H., Herrmann, J. L.
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology 2003
Fag:
Mycobacteriology and Aerobic Actinomycetes
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC156509/
https://ncbi.nlm.nih.gov/pubmed/12791844
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.41.6.2323-2329.2003
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