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Molecular-Beacon Multiplex Real-Time PCR Assay for Detection of Vibrio cholerae

A multiplex real-time PCR assay was developed using molecular beacons for the detection of Vibrio cholerae by targeting four important virulence and regulatory genes. The specificity and sensitivity of this assay, when tested with pure culture and spiked environmental water samples, were high, surpa...

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Bibliografiske detaljer
Main Authors: Gubala, Aneta J., Proll, David F.
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology 2006
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1563670/
https://ncbi.nlm.nih.gov/pubmed/16957277
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.02597-05
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