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Rapid Quantitative Detection of Lactobacillus sakei in Meat and Fermented Sausages by Real-Time PCR

A quick and simple method for quantitative detection of Lactobacillus sakei in fermented sausages was successfully developed. It is based on Chelex-100-based DNA purification and real-time PCR enumeration using a TaqMan fluorescence probe. Primers and probes were designed in the L. sakei 16S-23S rRN...

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Detalhes bibliográficos
Main Authors: Martín, Belén, Jofré, Anna, Garriga, Margarita, Pla, Maria, Aymerich, Teresa
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2006
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1563650/
https://ncbi.nlm.nih.gov/pubmed/16957227
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.02852-05
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