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Mimicking natural evolution in vitro: An N-acetylneuraminate lyase mutant with an increased dihydrodipicolinate synthase activity

N-acetylneuraminate lyase (NAL) and dihydrodipicolinate synthase (DHDPS) belong to the NAL subfamily of (β/α)(8)-barrels. They share a common catalytic step but catalyze reactions in different biological pathways. By rational design, we have introduced various mutations into the NAL scaffold from Es...

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Udgivet i:Proc Natl Acad Sci U S A
Principais autores: Joerger, Andreas C., Mayer, Sebastian, Fersht, Alan R.
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 2003
Fag:
Biological Sciences
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC156263/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12711733/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.0531477100
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