Incorporation of reporter molecule-labeled nucleotides by DNA polymerases. I. Chemical synthesis of various reporter group-labeled 2′-deoxyribonucleoside-5′-triphosphates

Fluorescent-labeled DNA is generated through enzymatic incorporation of fluorophore-linked 2′-deoxyribonucleoside-5′-triphosphates (dNTPs) by DNA polymerases. We describe the synthesis of a variety of dye-labeled dNTPs. Amino-linker-modified 5′-triphosphates of all four naturally occurring nucleobas...

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Bibliografiske detaljer

Udgivet i:	Nucleic Acids Res
Principais autores:	Giller, Gerald, Tasara, Taurai, Angerer, Bernhard, Mühlegger, Klaus, Amacker, Mario, Winter, Holger
Format:	Artigo
Sprog:	Inglês
Udgivet:	Oxford University Press 2003
Fag:	Articles
Online adgang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC156051/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12736313/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gkg370
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