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LeCPK1, a Calcium-Dependent Protein Kinase from Tomato. Plasma Membrane Targeting and Biochemical Characterization

The cDNA of LeCPK1, a calcium-dependent protein kinase, was cloned from tomato (Lycopersicon esculentum Mill.). LeCPK1 was expressed in Escherichia coli and purified from bacterial extracts. The recombinant protein was shown to be a functional protein kinase using a synthetic peptide as the substrat...

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Bibliografiske detaljer
Main Authors: Rutschmann, Frank, Stalder, Urs, Piotrowski, Markus, Oecking, Claudia, Schaller, Andreas
Format: Artigo
Sprog:Inglês
Udgivet: American Society of Plant Physiologists 2002
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC155880/
https://ncbi.nlm.nih.gov/pubmed/12011347
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1104/pp.000869
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