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Immunization of mouse spleen cell cultures in the absence of serum and its proteins using SiO2 and 2-mercaptoethanol.

High recoveries of plaque-forming cells were obtained in a serum-free medium using 2-mercaptoethanol and SiO2. Under such conditions an increase in the number of plaque-forming cells occurred between the fourth and fifth day of incubation, as compared with cultures with foetal calf serum. The additi...

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Detalhes bibliográficos
Autor principal: Burger, M
Formato: Artigo
Idioma:Inglês
Publicado em: 1982
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1555262/
https://ncbi.nlm.nih.gov/pubmed/6277774
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