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Quantification of antibody-secreting lymphocytes that react with Pf155/RESA from Plasmodium falciparum: an ELISPOT assay for field studies.

We have adapted the enzyme-linked immunospot assay (ELISPOT) to enumerate the cells from Plasmodium falciparum-primed donors that produce IgG in vitro in response to malaria antigens. In vitro activation of cell cultures with two synthetic peptides (EENVEHDA)4, and (LGRSGGDIIKMQTL) corresponding to...

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Autors principals: Fievet, N, Chougnet, C, Dubois, B, Deloron, P
Format: Artigo
Idioma:Inglês
Publicat: 1993
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC1554649/
https://ncbi.nlm.nih.gov/pubmed/8419087
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