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PPARγ knockdown by engineered transcription factors: exogenous PPARγ2 but not PPARγ1 reactivates adipogenesis

To determine functional differences between the two splice variants of PPARγ (γ1 and γ2), we sought to selectively repress γ2 expression by targeting engineered zinc finger repressor proteins (ZFPs) to the γ2-specific promoter, P2. In 3T3-L1 cells, expression of ZFP55 resulted in >50% reduction in γ...

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Udgivet i:Genes Dev
Principais autores: Ren, Delin, Collingwood, Trevor N., Rebar, Edward J., Wolffe, Alan P., Camp, Heidi S.
Format: Artigo
Sprog:Inglês
Udgivet: Cold Spring Harbor Laboratory Press 2002
Fag:
Research Communication
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC155307/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11782442/
https://ncbi.nlm.nih.govhttps://doi.org/10.1101/gad.953802
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